Functional characteristics of lactobacilli from traditional Bulgarian fermented milk products.

After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host.

Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of action (up to 24 h of incubation) were designed.

Six of the strains studied were found to show strain-specific survival capacity with low viability in conditions simulating stomach acidity and high resistance to bile and intestinal juices. In addition, the adherence capability (autoaggregation and hydrophobicity) of the strains was determined.

Obtained results allowed to select Lactobacillus strains with high survival ratios while passing through the GIT and good adherence properties, which make them suitable for the development of new probiotics.

Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals.

Our objective was to investigate the plasmid replicon-types involved in spread of ESBLs among Bulgarian Klebsiella pneumoniae and Escherichia coli. Sixty-three isolates, with transferable beta-lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL-, RAPD-, and plasmid replicon-type.

Phylogenetic typing and screening for the O25b-ST131 lineage were carried out for E. coli. The predominant ESBLs were CTX-M-15 (81%) among E. coli and CTX-M-3 (58%) among K. pneumoniae. Other sporadically found ESBLs were SHV-12 and TEM-139, and for the first time in Bulgaria, CTX-M-1 and CTX-M-14. Replicon typing revealed that plasmids carrying blaCTX-M-3 exclusively belonged to IncL/M-type, while blaCTX-M-15 was predominantly (94%) associated with IncF-type plasmids.

Annexin V

Annv Clemente Associates LLC 2 ML 367.5 EUR

Annexin V

E8ET1702-62 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Annexin V, Biotin conjugate

29013 Biotium 0.5mL 368.4 EUR
Description: Minimum order quantity: 1 unit of 0.5mL

Annexin V, Biotin Conjugate

29013-1 Biotium EA 309 EUR

Annexin V 647

E16FXP023a-100T EnoGene 100 tests 433.33 EUR
Description: Available in various conjugation types.

Annexin A5/Annexin V Rabbit mAb

A9262 Abclonal 200μL 213.2 EUR
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1).

Annexin A5 (Annexin V) (VAC-alpha)

MC-250 Kamiya Biomedical Company VAA-33 Ask for price

Annexin V (PE)

abx090613-100tests Abbexa 100 tests 644.4 EUR

Annexin V PE

EXB0027 ExBio 100 tests 244.8 EUR

Annexin V (APC)

abx090615-100tests Abbexa 100 tests 718.8 EUR

Annexin V APC

EXB0028 ExBio 100 tests 244.8 EUR

Annexin V (FITC)

abx090611-100tests Abbexa 100 tests 510 EUR

Annexin V FITC

EXB0024 ExBio 100 tests 244.8 EUR

Annexin V- FITC

E16FXP018a-100T EnoGene 100 tests 400 EUR
Description: Available in various conjugation types.

Annexin V, CF770

29046 Biotium 25ug 410.4 EUR
Description: Minimum order quantity: 1 unit of 25ug

Annexin V siRNA

20-abx907673 Abbexa
  • 661.20 EUR
  • 878.40 EUR
  • 15 nmol
  • 30 nmol

Annexin V siRNA

20-abx907674 Abbexa
  • 661.20 EUR
  • 878.40 EUR
  • 15 nmol
  • 30 nmol

Annexin V siRNA

20-abx900343 Abbexa
  • 661.20 EUR
  • 878.40 EUR
  • 15 nmol
  • 30 nmol

Annexin V, Human

E34M143H EnoGene 5 μg 155 EUR

Annexin V, sulforhodamine 101 conjugate (Texas-Red Annexin V)

29002 Biotium 0.5ML 332.4 EUR
Description: Minimum order quantity: 1 unit of 0.5ML

Annexin V antibody

10R-7939 Fitzgerald 100 ug 516 EUR
Description: Mouse monoclonal Annexin V antibody

Annexin V antibody

20R-1522 Fitzgerald 100 ug 781.2 EUR
Description: Rabbit polyclonal Annexin V antibody

Annexin V antibody

70R-11770 Fitzgerald 100 ug 632.4 EUR
Description: Rabbit polyclonal Annexin V antibody

Annexin V Antibody

49376-100ul SAB 100ul 399.6 EUR

Annexin V Antibody

49376-50ul SAB 50ul 286.8 EUR

Annexin V antibody

70R-13691 Fitzgerald 100 ug 386.4 EUR
Description: Affinity purified Rabbit polyclonal Annexin V antibody

Annexin V Antibody

3357-100 Biovision each 464.4 EUR

Annexin V Antibody

3357-30T Biovision each 175.2 EUR

Annexin V Antibody

F49706-0.08ML NSJ Bioreagents 0.08 ml 140.25 EUR
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Annexin V Antibody

F49706-0.4ML NSJ Bioreagents 0.4 ml 322.15 EUR
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Annexin V Antibody

F49707-0.08ML NSJ Bioreagents 0.08 ml 140.25 EUR
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]

Annexin V Antibody

F49707-0.4ML NSJ Bioreagents 0.4 ml 322.15 EUR
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]

Annexin V antibody

PAab09988 Lifescience Market 100 ug 463.2 EUR

Annexin V Antibody

RQ5261 NSJ Bioreagents 100ul 356.15 EUR
Description: The protein encoded by the ANXA5 gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. [RefSeq]

Annexin V Antibody

DF6580 Affinity Biosciences 100ul 280 EUR
Description: Human,Mouse,Rat

Annexin V Antibody

R30176 NSJ Bioreagents 100 ug 356.15 EUR
Description: Annexin V is also know as endonnexin II (ENX2),or placental protein 4 (PP4). It is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. Annexin V has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. It is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Annexin V Antibody

R31475 NSJ Bioreagents 100 ug 356.15 EUR
Description: Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Annexin V antibody

CAF50033-100ug Biomatik Corporation 100ug 312 EUR

Annexin V antibody

CAF50425-100ug Biomatik Corporation 100ug 338 EUR

Annexin V Antibody

E2300787 EnoGene 100ug 225 EUR
Description: Available in various conjugation types.

Annexin V Antibody

E300518 EnoGene 100ug/200ul 275 EUR
Description: Available in various conjugation types.

Annexin V antibody

E39-09988 EnoGene 100ug/100ul 225 EUR
Description: Available in various conjugation types.

Annexin V Antibody

DF6580-100ul Affinity Biosciences 100ul 280 EUR

Annexin V Antibody

DF6580-200ul Affinity Biosciences 200ul 350 EUR

Annexin V Antibody

49376 SAB 100ul 499 EUR

Annexin V, Recombinant

20014 AAT Bioquest 100 ug 222 EUR

Annexin V, Recombinant

20015 AAT Bioquest 1 mg 1509 EUR

Annexin V, Recombinant

20014-100ug AAT Bioquest 100 ug 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V, Recombinant

20015-1mg AAT Bioquest 1 mg 1509 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V (ANXA5) Antibody (Biotin)

abx414512-100tests1ml Abbexa 100 tests (1 ml) 610.8 EUR

Annexin V ELISA kit

55R-IB49646 Fitzgerald 96 wells 1413.6 EUR
Description: ELISA kit for the detection of Annexin V in the research laboratory

Annexin V ELISA kit

55R-ORG643 Fitzgerald 96 wells 541.2 EUR
Description: ELISA kit for the detection of Annexin V in the research laboratory

Annexin V Mouse mAb

E2221242 EnoGene 100ul 225 EUR
Description: Available in various conjugation types.

Annexin V Dyomics 647

EXB0023 ExBio 100 tests 266.4 EUR

Annexin V Rabbit pAb

E2381706 EnoGene 100ul 225 EUR
Description: Available in various conjugation types.

Annexin V Rabbit pAb

E2382841 EnoGene 100ul 225 EUR
Description: Available in various conjugation types.

Annexin V Rabbit mAb

E2R22841 EnoGene 100ul 255 EUR
Description: Available in various conjugation types.

Annexin V Rabbit mAb

52083 SAB 100ul 499 EUR

Annexin V Rabbit mAb

59326 SAB 100ul 339 EUR

Annexin V Rabbit pAb

27222 SAB 100ul 319 EUR

Annexin V-PE Reagent

1014-1000 Biovision each 1358.4 EUR

Annexin V-PE Reagent

1014-200 Biovision each 483.6 EUR

Annexin V-PE Reagent

E-CK-A115-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-PE Reagent

E-CK-A115-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-PE Reagent

E-CK-A115-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-PE Reagent

E-CK-A115-50Tests Elabscience Biotech 50 Tests 110 EUR

Recombinant human Annexin A5/Annexin V/ANXA5 Protein

RP01180 Abclonal 10 μg 186 EUR

Active Annexin V (ANXA5)

4-APA259Ra01 Cloud-Clone
  • 743.62 EUR
  • 322.80 EUR
  • 2458.56 EUR
  • 899.52 EUR
  • 1679.04 EUR
  • 573.60 EUR
  • 5966.40 EUR
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Annexin V expressed in: E.coli

Annexin V-Cy5 Reagent

1013-1000 Biovision each 1358.4 EUR

Annexin V-Cy5 Reagent

1013-200 Biovision each 483.6 EUR

Annexin V-Cy3 Reagent

1002-1000 Biovision each 1358.4 EUR

Annexin V-Cy3 Reagent

1002-200 Biovision each 483.6 EUR

Annexin V, FITC Labeled

20030 AAT Bioquest 100 tests 222 EUR

Active Annexin V (ANXA5)

RPU55516-100ug Biomatik Corporation 100ug 918.5 EUR

Active Annexin V (ANXA5)

RPU55516-1mg Biomatik Corporation 1mg 3619.2 EUR

Active Annexin V (ANXA5)

RPU55516-50ug Biomatik Corporation 50ug 556.8 EUR

Annexin V, FITC Labeled

20030-100tests AAT Bioquest 100 tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-APC Reagent

E-CK-A117-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-APC Reagent

E-CK-A117-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-APC Reagent

E-CK-A117-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-APC Reagent

E-CK-A117-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-EGFP Reagent

1004-1000 Biovision each 1358.4 EUR

Annexin V-EGFP Reagent

1004-200 Biovision each 483.6 EUR

Annexin V-FITC Reagent

1001-1000 Biovision each 1495.2 EUR

Annexin V-FITC Reagent

1001-200 Biovision each 529.2 EUR

anti- Annexin V antibody

FNab09988 FN Test 100µg 658.5 EUR
Description: Antibody raised against Annexin V

anti- Annexin V antibody

FNab00436 FN Test 100µg 606.3 EUR
Description: Antibody raised against Annexin V

anti- Annexin V antibody

FNab00437 FN Test 100µg 606.3 EUR
Description: Antibody raised against Annexin V

Annexin V Binding Assay

TBS2108 Tribioscience 100 tests 200 EUR

Annexin V, TRITC Labeled

20031-100tests AAT Bioquest 100 tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-FITC Reagent

E-CK-A111-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-FITC Reagent

E-CK-A111-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-FITC Reagent

E-CK-A111-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-FITC Reagent

E-CK-A111-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-EGFP Reagent

E-CK-A119-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-EGFP Reagent

E-CK-A119-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-EGFP Reagent

E-CK-A119-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-EGFP Reagent

E-CK-A119-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-HRP Antibody

E2700787 EnoGene 100ug 225 EUR
Description: Available in various conjugation types.

OPSB00056-100T - Aposcreen Annexin V : Biotin

OPSB00056-100T Aviva Systems Biology 100Tests 389 EUR

Annexin V (ANXA5) Antibody

abx230436-100ug Abbexa 100 ug 577.2 EUR

Annexin V (ANXA5) Antibody

abx230437-100ug Abbexa 100 ug 577.2 EUR

Annexin V (ANXA5) Antibody

20-abx109540 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Annexin V (ANXA5) Antibody

20-abx121016 Abbexa
  • 360.00 EUR
  • 526.80 EUR
  • 226.80 EUR
  • 100 ul
  • 200 ul
  • 30 ul

Annexin V (ANXA5) Antibody

abx412125-01mg Abbexa 0.1 mg 1078.8 EUR

Annexin V (ANXA5) Antibody

20-abx109257 Abbexa
  • 493.20 EUR
  • 2214.00 EUR
  • 718.80 EUR
  • 218.40 EUR
  • 360.00 EUR
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Annexin V Binding Buffer

abx090621-100tests Abbexa 100 tests 343.2 EUR

Annexin V (ANXA5) Antibody

20-abx110998 Abbexa
  • 878.40 EUR
  • 477.60 EUR
  • 150 ul
  • 50 ul

Annexin V (ANXA5) Antibody

abx032878-400ul Abbexa 400 ul 627.6 EUR

Annexin V (ANXA5) Antibody

abx032878-80l Abbexa 80 µl 343.2 EUR

Annexin V (ANXA5) Antibody

abx032879-400ul Abbexa 400 ul 627.6 EUR

Annexin V (ANXA5) Antibody

abx032879-80l Abbexa 80 µl 343.2 EUR

Annexin V (ANXA5) Antibody

abx037408-100ug Abbexa 100 ug 469.2 EUR

Annexin V (ANXA5) Antibody

abx038319-100ug Abbexa 100 ug 469.2 EUR

Annexin V (ANXA5) Antibody

abx414511-01mg Abbexa 0.1 mg 526.8 EUR

Annexin V, FITC conjugate

29001 Biotium 0.5ML 332.4 EUR
Description: Minimum order quantity: 1 unit of 0.5ML

Annexin V (ANXA5) Antibody

20-abx001443 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 218.40 EUR
  • 376.80 EUR
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Annexin V, FITC Conjugate

29001-1 Biotium EA 274 EUR

Annexin V-Cy3 conjugate

20065-100Tests AAT Bioquest 100 Tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-Cy5 conjugate

20066-100Tests AAT Bioquest 100 Tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-Cy5.5 conjugate

20067-100Tests AAT Bioquest 100 Tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-Cy7 conjugate

20068-100Tests AAT Bioquest 100 Tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Annexin V-AF647 Reagent

E-CK-A113-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-AF647 Reagent

E-CK-A113-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-AF647 Reagent

E-CK-A113-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-AF647 Reagent

E-CK-A113-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-EV450 Reagent

E-CK-A133-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-EV450 Reagent

E-CK-A133-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-EV450 Reagent

E-CK-A133-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-EV450 Reagent

E-CK-A133-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-EV500 Reagent

E-CK-A135-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-EV500 Reagent

E-CK-A135-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-EV500 Reagent

E-CK-A135-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-EV500 Reagent

E-CK-A135-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-AF488 Reagent

E-CK-A137-100T Elabscience Biotech 100T 190 EUR

Annexin V-AF488 Reagent

E-CK-A137-200T Elabscience Biotech 200T 298 EUR

Annexin V-AF488 Reagent

E-CK-A137-500T Elabscience Biotech 500T 660 EUR

Annexin V-AF488 Reagent

E-CK-A137-50T Elabscience Biotech 50T 110 EUR

Annexin V-ER780 Reagent

E-CK-A139-100Tests Elabscience Biotech 100 Tests 190 EUR

Annexin V-ER780 Reagent

E-CK-A139-200Tests Elabscience Biotech 200 Tests 298 EUR

Annexin V-ER780 Reagent

E-CK-A139-500Tests Elabscience Biotech 500 Tests 660 EUR

Annexin V-ER780 Reagent

E-CK-A139-50Tests Elabscience Biotech 50 Tests 110 EUR

Annexin V-Biotin Reagent

1003-100 Biovision each 444 EUR

Annexin V-Biotin Reagent

1003-1000 Biovision each 2865.6 EUR

Among E. coli, 59% of the isolates were clonally related. Isolates of that cluster produced CTX-M-15, belonged to the O25b-ST131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. Among CTX-M-3-producing K. pneumoniae, two prevailing RAPD-types were found, one remained restricted to one centre and the second was found in three centres.

The incompatibility groups IncN and IncA/C linked with blaSHV-12 respectively blaTEM-139 were found only once. To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among Bulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX-M-15-producing E. coli and IncL/M plasmids among CTX-M-3 positive K. pneumoniae isolates.